Journal: Scientific Reports

Article Title: Transcriptome responses in alfalfa associated with tolerance to intensive animal grazing

doi: 10.1038/srep19438

Figure Lengend Snippet: Primers used in real-time RT-PCR analysis of 12 grazing upregulated transcripts, as identified from the transcriptome analysis, and two reference genes UBL-2a and ADF.

Article Snippet: To validate those results, real-time RT-PCR analysis was subsequently performed on an Opticon II system (MJ Research, Waltham, MA, USA), with SYBR® green reagents (Bio-Rad, Hercules, CA) in a 20 μl reaction volume that contained 250 nM transcript-specific primer pairs ( ).

Techniques: Quantitative RT-PCR

Journal: Nucleic Acids Research

Article Title: RDML: structured language and reporting guidelines for real-time quantitative PCR data

doi: 10.1093/nar/gkp056

Figure Lengend Snippet: Real-time PCR data exchange with RDML. RDML acts as a facilitator of, and common language for exchange of qPCR data and related information between users, software programs and databases.

Article Snippet: The RDML project is supported by companies providing real-time PCR instruments and reagents (Bio-Rad Laboratories, Roche, Applied Biosystems) or reagents only (Eurogentec, Primer Design), developing PCR data analysis software (Biogazelle, LabonNet and MultiD Analysis) and organizing qPCR courses (Biogazelle, TATAA Biocenter), as well as a number of opinion leaders, core facility managers and researchers active in the field of qPCR, including the developers of geNorm, qBase, RTprimerDB and REST.

Techniques: Real-time Polymerase Chain Reaction, Software